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AMPLIFICATION PRIMERS WITH NON-STANDARD BASES FOR INCREASED REACTION SPECIFICITY
AMPLIFICATION PRIMERS WITH NON-STANDARD BASES FOR INCREASED REACTION SPECIFICITY
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机译:具有非标准碱的扩增试剂,可提高反应特异性
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摘要
Described herein are methods for increasing the annealing specificity of an amplification reaction using Iso-base Amplification Primers ("IAPs"). IAPs containing an iso-region are capable of regulating sequence-specific annealing thereby enhancing primer-template hybridization for sequence-specific amplification of nucleotides.
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