Nucleic acid detection method, capture probe, detection probe set, microarray, nucleic acid detection kit, nucleic acid-immobilized solid phase, and fluidic device

摘要

A nucleic acid detection method comprising the following steps. a) a solution containing a detection probe labeled with a labeling substance and a nucleic acid, a first portion comprising a sequence capable of hybridizing with the nucleic acid, and a second portion comprising a sequence capable of hybridizing with the detection probe; A step of contacting a solid phase to which a capture probe having a stem part that forms a double strand is fixed, b) a nucleic acid hybridizing to the first part, and a detection probe hybridizing to the second part A step of forming a nucleic acid-detection probe-capture probe complex on the solid phase, c) a step of ligating the nucleic acid and the detection probe, and d) a binding between the detection probe that is not ligated with the nucleic acid and the capture probe. E) a step of detecting a labeling substance in the complex