Sensitive, Selective Fluorescent Probes for the Detection and Identification of Nucleic Acids

摘要

The long-term goals of this project are to develop new strategies for the use of oligonucleotides tagged with multiple fluorescent labels as hybridization probes of specific nucleic acid sequences. First, we have designed (and continue to design) new classes of fluorescent labels for DNA. Second, we have developed novel modes of interaction between fluorescent labels, leading to color changes on detection of nucleic acids. Finally, we have developed new methods for increasing specificity of nucleic acid recognition.