Nanofluidic-based fluorescence near-field microscopes are provided. The present fluorescence near-field microscope is configured to concentrate an incident light source in a narrow space, to change a quantum yield of a fluorescent sample existing in the narrow space, and to concentrate a fluorescent signal output generated in the narrow space in a specific direction. And a nanopore or nanochannel which is connected to the narrow space of the nano antenna and provides a moving path for introducing the fluorescent sample into the narrow space. Thus, it is possible to concentrate the incident light source in a specific narrow space of the nano-antenna, increase the low quantum yield of the fluorescent sample, efficiently detect the fluorescence signal output in a specific direction, enable high signal-to-noise ratio and high-resolution fluorescence detection, By introducing a fluorescent sample through a pore or a nanochannel to the nanotenna, the sample can be scanned without mechanical movement of the nanotenna. The nanofluidics-based fluorescence near-field microscope can perform DNA sequence analysis by linearly passing the fluorescence-labeled DNA through the nanopore or nanochannel and reading the fluorescence signal sequentially generated in the narrow space. ;
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