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COMPOSITIONS AND METHODS FOR USE IN A PCR ASSAY FOR DETERMINING THE GENOTYPE AND VIRAL LOAD FOR RESPIRATORY SYNCYTIAL VIRUS
COMPOSITIONS AND METHODS FOR USE IN A PCR ASSAY FOR DETERMINING THE GENOTYPE AND VIRAL LOAD FOR RESPIRATORY SYNCYTIAL VIRUS
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机译:用于PCR鉴定呼吸道合胞病毒基因型和病毒载量的组合物和方法。
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摘要
Methods are provided for a sensitive and specific assay for the determination of viral load and genotyping of RSV in a biological sample. Compositions and kits for use in the methods also are provided, including optimized primers for the amplification of and detection of the RSV open reading frames from subtypes A and B, and probes for distinguishing between the subtypes. Also provided are methods for amplifying and sequencing an open reading from of an RSV F protein, and compositions and kits for use in the methods.
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