Intracellular delivery of biomolecules mediated by pored surfaces

摘要

The present disclosure is a method and device for delivering a compound into a cell comprising passing a cell suspension through a surface containing the pore, wherein the pore deforms the cell, thereby It relates to methods and devices that cause perturbation of the cell such that the compound enters the cell and the cell suspension is in contact with the compound. The surface can be a filter, membrane, or microsieve. Cell perturbations can be cell breaks that allow extracellular material to migrate into the cell.