bacterial cell packaging system, method for packaging a reporter nucleic acid molecule, composition and method for detecting a bacterial cell presence or absence in a sample

摘要

abstract “bacterial cell packaging system, method for packaging a reporter nucleic acid molecule, composition, method for detecting a bacterial cell presence or absence in a sample, vector, method for detecting a target transcript in a cell, Methods for Detecting the Presence of a Bacterial Cell in a Sample and Kit ”Methods and systems for packaging reporter nucleic acid molecules into the non-replicative transduction particles for use as reporter molecules are provided. non-replicative transduction particles can be constructed from viruses and use viral transduction systems and replication systems. reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. The methods and systems are provided for detecting target nucleic acid cells and molecules using non-replicative transduction particles as reporter molecules.