cationic liposome vehicle dna vaccine formulation useful for maximizing the potency of the leishmaniasis vaccine

摘要

The present invention describes the vaccination of DNA with plasmid encoding the leishmania donovani cysteine protease c (cpc) gene to induce effective protection against visceral leishmaniasis (vl). The DNA vaccine enclosed in the cationic liposomes comprises a pvax1 plasmid vector encoding the 1 cpc gene. donovani operably linked to transcriptional regulatory sequences. The invention relates to the efficacy of the liposomal formulation containing the bilayer-associated mpla adjuvant, which encapsulates the dv pvax1-cpc construct, against the lethal 1-infection model. donovani in balb / c mice. This liposome DNA vaccine formulation is highly effective in inducing a robust cell-mediated immune response against leishmaniasis parasites upon intramuscular administration.