首页> 外国专利> A DNA VACCINE FORMULATION IN CATIONIC LIPOSOME VEHICLE USEFUL FOR MAXIMIZING VACCINE POTENCY FOR LEISHMANIASIS

A DNA VACCINE FORMULATION IN CATIONIC LIPOSOME VEHICLE USEFUL FOR MAXIMIZING VACCINE POTENCY FOR LEISHMANIASIS

机译：阳离子脂质体车辆中的DNA疫苗配方，可最大程度地提高利什曼病的免疫力

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摘要

The present invention discloses DNA vaccination with plasmid encoding the cysteine protease C (cpc) gene of Leishmania donovani to induce effective protection against visceral leishmaniasis (VL). The DNA vaccine entrapped in the cationic liposomes comprises of a plasmid vector pVAX1 encoding L. donovani cpc gene operably linked to transcriptional regulatory sequences. The invention relates to efficacy of liposomal formulation containing the bilayer-associated adjuvant MPLA, encapsulating the DNA construct pVAX1-cpc, against lethal L. donovani infection model in BALB/c mice. This formulation of liposomal DNA vaccine is highly effective in inducing robust cell-mediated immune response against leishmanial parasites upon intramuscular administration. 展开▼

机译：本发明公开了用编码利什曼原虫（Leishmania donovani）的半胱氨酸蛋白酶C（cpc）基因的质粒进行DNA疫苗接种，以诱导针对内脏利什曼病（VL）的有效保护。包埋在阳离子脂质体中的DNA疫苗包含一个质粒载体pVAX1，该质粒编码可操纵地连接至转录调控序列的多诺尼乳酸菌cpc基因。本发明涉及包封了DNA构建体pVAX1-cpc的包含双层相关佐剂MPLA的脂质体制剂对BALB / c小鼠中的致死性多巴氏乳杆菌感染模型的功效。脂质体DNA疫苗的这种制剂在肌肉内施用时在诱导针对利什曼原虫的强大的细胞介导的免疫应答中非常有效。展开▼

著录项

公开/公告号WO2016103273A1

专利类型

公开/公告日2016-06-30

原文格式PDF

申请/专利权人 COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH;
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申请/专利号WO2015IN00186

发明设计人 ALI NAHID;DAS AMRITA;
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申请日2015-04-28

分类号A61K39/008;A61K39/39;

国家 WO

入库时间 2022-08-21 14:17:21

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1. 构建用于抗利什曼病和结核病的疫苗开发的DNA嵌合体 [P] . 中国专利： CN101896196A . 2010-11-24

2. 提高免疫力的中药提取物组合物、其制备方法及其在制备提高免疫力药物中的应用 [P] . 中国专利： CN107661461B . 2020.10.02

3. cationic liposome vehicle dna vaccine formulation useful for maximizing the potency of the leishmaniasis vaccine [P] . BR112017013623A2 . 2018-03-13

机译：阳离子脂质体载体dna疫苗制剂，可用于最大化利什曼病疫苗的效力

4. A DNA VACCINE FORMULATION IN CATIONIC LIPOSOME VEHICLE USEFUL FOR MAXIMIZING VACCINE POTENCY FOR LEISHMANIASIS [P] . IN2014DE03864A . 2016-06-24

机译：阳离子脂质体车辆中的DNA疫苗配方，可最大程度地提高利什曼病的疫苗强度

5. cationic liposome stabilization method in aqueous formulations, liposome product, vaccine adjuvant, chlamydia, malaria or tuberculosis vaccine and delivery system [P] . BRPI0512757A . 2008-04-08

机译：水性制剂，脂质体产品，疫苗佐剂，衣原体，疟疾或结核病疫苗和输送系统中阳离子脂质体的稳定化方法

1. Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice [J] . Sukhbir Kaur, Tejinder Kaur, Jyoti Joshi 生物医学研究杂志（英文版） . 2016,第004期

2. Comparison of BCG, MPL and cationic liposome adjuvant systems in leishmanial antigen vaccine formulations against murine visceral leishmaniasis [J] . Rajesh Ravindran, Sudipta Bhowmick, Amrita Das, BMC Microbiology . 2010,第1期

3. Enhanced potency of individual and bivalent DNA replicon vaccines or conventional DNA vaccines by formulation with aluminum phosphate. [J] . Yu YZ, Wang WB, Li N, Biologicals: Journal of the International Association of Biological Standardization . 2010,第6期

4. Cationic liposomes formulated with a novel whole Leishmania lysate (WLL) as a vaccine for leishmaniasis in murine model [J] . Jafari Iman, Shargh Vahid Heravi, Shahryari Maryam, Immunobiology: Zeitschrift fur Immunitatsforschung . 2018,第6a7期

5. Negatively charged carbon nanohorn supported cationic liposome nanoparticles: a nove1 l delivery vehicle for anti-nicotine vaccine [C] . Hong Zheng, Yun Hu, Wei Huang AIChE Annual Meeting . 2014

6. Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation. [D] . Tantituvanont, Angkana. 2003

7. Comparison of BCG MPL and cationic liposome adjuvant systems in leishmanial antigen vaccine formulations against murine visceral leishmaniasis [O] . Rajesh Ravindran, Sudipta Bhowmick, Amrita Das, 2010

8. Comparison of BCG, MPL and cationic liposome adjuvant systems in leishmanial antigen vaccine formulations against murine visceral leishmaniasis [O] . Rajesh Ravindran, Sudipta Bhowmick, Amrita Das, 2012

9. Therapeutic Potential of Liposomes as Carriers in Leishmaniasis, Malaria, and Vaccines. [R] . Alving, C. R. 1982

1. Targeting hepatitis B virus antigens to dendritic cells by heat shock protein to improve DNA vaccine potency [J] . Qin-Long Gu ,Xue Huang ,Wen-Hong Ren . 世界胃肠病学杂志：英文版 . 2007,第44期

2. Epitope DNA vaccines against tuberculosis： spacers and ubiquitin modulates cellular immune responses elicited by epitope DNA vaccine [J] . . 第二军医大学学报 . 2005,第2期

3. Comparative Study on the Immunogenicity between Hsp70 DNA Vaccine and Hsp65 DNA Vaccine in Human Mycobacterium Tuberculosis [J] . DAI Wuxing ,HUANG Hailang ,YUAN Ye . 华中科技大学学报（医学）（英德文版） . 2001,第003期

4. Comparative Study on the Immunogenicity between Hsp70 DNA Vaccine and Hsp65 DNA Vaccine in Human Mycobacterium Tuberculosis [J] . DAI ,Wuxing ,HUANG . 华中科技大学学报：医学英德文版 . 2001,第003期

5. 内含子A增强Hsp65在真核细胞中的表达效率及提高DNA疫苗在小鼠中的免疫原性 [C] . 吴娟 ,马辉 ,范小勇 . 中华医学会结核病学分会2011年学术会议 . 2011

6. 用于防龋DNA疫苗粘膜给药的阳离子脂质体载体的研究 [A] . 奚颖霞 . 2009