首页> 外国专利> A DNA VACCINE FORMULATION IN CATIONIC LIPOSOME VEHICLE USEFUL FOR MAXIMIZING VACCINE POTENCY FOR LEISHMANIASIS

A DNA VACCINE FORMULATION IN CATIONIC LIPOSOME VEHICLE USEFUL FOR MAXIMIZING VACCINE POTENCY FOR LEISHMANIASIS

机译：阳离子脂质体车辆中的DNA疫苗配方，可最大程度地提高利什曼病的疫苗强度

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摘要

The present invention discloses DNA vaccination with plasmid encoding the cysteine protease C (cpc) gene of Leishmania donovani to induce effective protection against visceral leishmaniasis (VL). The DNA vaccine entrapped in the cationic liposomes comprises of a plasmid vector pVAXl encoding L. donovani cpc gene operably linked to transcriptional regulatory sequences. The invention relates to efficacy of liposomal formulation containing the bilayer-associate,d adjuvant MPLA, encapsulating the DNA construct pVAX1- cpc, against lethal L. donovani infection model in BALBIc mice. This formulation of liposomal DNA vaccine is highly effective in inducing robust cell-mediated immune response against leishmania1 parasites upon intramuscular administration.

机译：本发明公开了用编码利什曼原虫（Leishmania donovani）的半胱氨酸蛋白酶C（cpc）基因的质粒进行DNA疫苗接种，以诱导针对内脏利什曼病（VL）的有效保护。包埋在阳离子脂质体中的DNA疫苗包含质粒载体pVAX1，该质粒载体pVAX1编码可与转录调控序列有效连接的多诺氏乳杆菌cpc基因。本发明涉及包封DNA构建体pVAX1-cpc的包含双层相关的d佐剂MPLA的脂质体制剂对BALBIC小鼠的致死性多诺尼氏菌感染模型的功效。脂质体DNA疫苗的这种制剂在肌肉内给药后可有效诱导针对利什曼原虫寄生虫的强大的细胞介导的免疫反应。

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公开/公告号IN2014DE03864A

专利类型
公开/公告日2016-06-24

原文格式PDF
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申请/专利号IN3864/DEL/2014
发明设计人 NAHID ALI;AMRITA DAS;
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申请日2014-12-23
分类号A23J3/10;
国家 IN
入库时间 2022-08-21 14:25:25

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