Method for the viability of embryo in white fertilized egg using hyperspectral imaging

摘要

The present invention relates to a method for determining whether or not an embryo survives in a turtle using ultra-spectroscopic images, and more particularly, to a method for determining whether embryos are viable or not by incubating a virus in a turtle egg and culturing, obtaining and purifying the same, In order to discriminate non-destructively, we illuminate the inside with halogen light, apply ultrasound imaging system to find the wavelength that can best confirm the contents, and detect the flow of live turbulence through line scan of hyperspectral image, We applied the CLAHE image processing method, the Gabor filter, and the texture analysis method of the image to the ultrasound image which can be used to massively evaluate the embryo survival inside the fertilized egg in real time The present invention relates to a method for discriminating the presence or absence of embryo survival. (A) imaging an ultraspectral transmission image of 400 to 1000 nm from a flow field irradiated with halogen illumination with an electron multiplying chargecoupled device (EMCCD) camera; (B) obtaining an image expressing the inside of the turbulence through statistical analysis processing (PCA) on the image photographed by the camera, performing a preprocessing method for obtaining a clear image, and determining whether or not to survive through texture analysis .