Method for suppressing dedifferentiation of cells susceptible to dedifferentiation, method for preparing the cells, and method for producing substance

摘要

The present invention is a method for suppressing the dedifferentiation of cells susceptible to dedifferentiation, which comprises the steps of (1) applying the cells to a polymer porous membrane, and (2) culturing and proliferating the cells. Here, the polymer porous film has a three-layer structure polymer having a surface layer A and a surface layer B having a plurality of pores, and a macrovoid layer sandwiched between the surface layer A and the surface layer B. The porous membrane, wherein the average pore diameter of the pores present in the surface layer A is smaller than the average pore diameter of the pores present in the surface layer B, and the macrovoid layer is bonded to the surface layers A and B And the plurality of macrovoids surrounded by the partition wall and the surface layers A and B, and the holes in the surface layers A and B communicate with the macrovoids.