Biocatalysts and methods for the hydroxylation of chemical compounds

摘要

A designed polypeptide having proline hydroxylase activity, comprising an amino acid sequence that has at least 80% sequence identity with SEQ ID NO: 2 in which proline hydroxylase has at least 1.2 times greater activity in comparison with the polypeptide of SEQ ID NO: 2 and in which the amino acid sequence comprises a residue difference compared to the sequence of SEQ ID NO: 2 at the position of residue X166.