Disclosed is a method for inducing artificial ovulation and spawning of freshwater eels to promote artificial ovulation, without employing intraperitoneal injection, by feeding a feminization-inducing material (e.g., 17²-estradiol) for inducing feminization during a predetermined period into a raising bath in which broodfish as a subject of artificial ovulation are raised and, when an ovulation induction period arrives, adding pituitary extracts of catadromous fish, i.e., a flathead mullet pituitary extract (FPE), as an artificial ovulation-promoting substance to the bath. Preferably, according to the present invention, raising water of the raising bath is prepared by selectively setting fresh water, brackish water and seawater conditions, a feminization-inducing material for inducing complete feminization of broodfish of the freshwater eels is fed to the raising bath including the raising water under a fresh water condition, and pre-treatment for feminization is performed. Subsequently, the raising water is set to a seawater condition and the ovulation-inducing substance is fed to the raising water, thereby inducing ovulation and spawning of the broodfish.
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