METHOD FOR PRODUCING TETRAHYDROPYRIMIDINE BY FERMENTING RECOMBINANT CORYNEBACTERIUM GLUTAMICUM

摘要

A method for producing tetrahydropyrimidine by fermenting recombinant Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum is obtained by overexpressing, in Corynebacterium glutamicum, an aspartokinase gene lysC of which feedback inhibition is relieved, then replacing a promoter of a dihydropyrimidine dicarboxylic acid synthetase in the recombinant bacterium to weaken the activity of the dihydropyrimidine dicarboxylic acid synthetase, and then transferring a tetrahydropyrimidine synthetic path related gene ectABC into the recombinant bacterium. The recombinant Corynebacterium glutamicum can be fermented using different cheap raw materials under a low salt condition to produce tetrahydropyrimidine, and cheap corn slurry can be also used as a nutritional component to replace expensive yeast powder, so that the costs of the raw materials are further reduced. In addition, the recombinant Corynebacterium glutamicum resolves the problem of biosafety, simplifies the post-extraction process, and has a good market application prospect.