AMYLASE MUTANT HAVING HIGH SPECIFIC ACTIVITY AND THERMAL STABILITY, GENE OF MUTANT, AND APPLICATIONS THEREOF

摘要

The present invention relates to the field of agricultural biotechnology and specifically relates to an amylase mutant having high specific activity and thermal stability, the gene of the mutant, and applications thereof. A wild-type amylase of which the amino acid sequence is as represented by SEQ ID NO. 1 undergoes an S33A/S34E/V35H point mutation and the amino acids at loci 178 and 179 are removed, thus acquiring the amylase mutant. Compared with the wild-type amylase, the amylase mutant of the present invention has the following traits: 1) enhanced amylase activity; and 2) enhanced amylase thermal stability.