RODENTS WITH CONDITIONAL ACVR1 MUTANT ALLELES

摘要

FIELD: biochemistry.;SUBSTANCE: invention relates to the field of biochemistry, in particular to a genetically modified rodent, capable of demonstrating the heterotopic ossification phenotype. Said rodent comprises an Acvr1 construct inside an allele, and said construct comprises the mutant exon 5 Acvr1 in antisense orientation, flanked by a pair of site-specific recombinase recognition sites (SRRS), and additionally comprises a second pair of SRRS, directing the removal of wild-type Acvr1 exon 5 under the action of inducible site-specific recombinase, and a gene encoding the inducible site-specific recombinase. Also disclosed is a mouse embryonic stem (ES) cell of said rodent and its use as a donor cell to obtain a mouse containing the genetically modified Acvr1 allele and the tamoxifen-induced Cre-ER^T or Cre-ER^T2 gene. Invention also relates to a method for producing a genetically modified mouse comprising the genetically modified Acvr1 allele and tamoxifen-induced Cre-ER^T or Cre-ER^T2.;EFFECT: invention makes it possible to efficiently obtain a genetically modified rodent demonstrating the heterotopic ossification phenotype.;8 cl, 12 dwg, 4 tbl