A method of evolution of a protein in an insect cell production host. The method includes steps of evolving a template protein to produce a set of mutant proteins in the insect cell production host; screening the set of mutant proteins for at least one predetermined property; characteristic or activity; selecting an up-mutant protein from the set of mutant proteins based upon the at least one predetermined property, characteristic or activity; and expressing the up-mutant protein in the same insect cell production host as used in the evolving step.
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