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TYPE I-B CRISPR-CAS SYSTEM GENE CAS3-BASED GENE EDITING METHOD
TYPE I-B CRISPR-CAS SYSTEM GENE CAS3-BASED GENE EDITING METHOD
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机译:I-B型CRISPR-CAS系统基因CAS3为基础的基因编辑方法
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摘要
A novel gene editing system developed based on the gene cas3 in the type I-B CRISPR-Cas system in the chromosome of Streptomyces virginiae IBL14 enables the gene editing of a type I CRISPR-Cas system on biological cell genomes for the first time. In the system, Cas3 can specifically cleave a DNA fragment of interest guided by crRNA or t-DNA. The new approach can be applied to rapid, simple and correct genome editing of prokaryotic and eukaryotic cells, which provides novel supplements and selections for the commercialized gene editing system developed based on Cas9 in type H.
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机译:一种新的基因编辑系统,它基于 Streptomyces virginiae I> IBL14染色体中IB CRISPR-Cas系统中的cas3基因开发,可对生物细胞基因组上的I CRISPR-Cas系统进行基因编辑,用于第一次。在该系统中,Cas3可以特异性切割由crRNA或t-DNA引导的目标DNA片段。该新方法可以应用于原核和真核细胞的快速,简单和正确的基因组编辑,这为基于H型Cas9开发的商业化基因编辑系统提供了新颖的补充和选择。
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