Nucleic acid molecule and application thereof in humanized antibody

摘要

The present invention provides a nucleic acid molecule, comprising an immunoglobulin gene or a fragment thereof. The nucleic acid molecule comprises an IgM gene (IgHCμ) and an IgM switch region (Sμ), and the Sμ and the IgHCμ are both host animal sequences. According to the present invention, a humanoral antibody is directly acquired, the artificial modification in the later period is reduced, and the druggability of the antibody is improved. The early development and maturation of transgenic B-cells and the number of the B-cells are completely consistent with those of normal mice, thereby facilitating the differentiation of the B-cells; the specificity and diversity of the produced antibody are improved; and the efficiency for screening the therapeutic antibody is improved.