Application of nucleic acid molecules in the preparation of nucleic acid molecules and humanized single domain antibodies

摘要

The present invention provides an application of nucleic acid molecules in the preparation of nucleic acid molecules and single domain antibodies.The nucleic acid molecule comprising the immunoglobulin gene of the host animal or its fragmentHost 5 igh5 - end enhancerIgM switch area μ） AndThe IgM arrayAll host animal IgM sequencesIgM deleted the ch1 sequenceIgG expression regulating element is derived from host animalsS γ、TM1Contains arrays of TM2 and Polya, etc.IgG C γ ArrayHuman sequences (CH2 and CH3) in human γ－ It is derived from dch1.The present invention is a human single domain antibody that ensures normal occurrence of B cells in transgenic animals and is expressed in transgenic animals, which reduces the artificial alteration in the later stages and enhances the likelihood of antibodies as a medicine.Diagram