Application of nucleic acid molecules in nucleic acid molecules and humanized antibodies

摘要

The present invention provides a nucleic acid molecule containing an immunoglobulin gene or its fragment, and IgM gene μ） And the IgM switch region μ） Including s μ And ighc μ Is characterized by a host animal sequence.The present invention can directly acquire complete human antibodies, reduce manual modification in late stages and increase the likelihood of antibodies as medicines.The early development and maturation of the B cell of the present invention and the number of B cells are completely consistent with the normal mouse, and the differentiation of B cells is promoted; Specificity and diversity of antibody production are increased; Improve the screening efficiency of therapeutic antibodies.Diagram