首页> 外国专利> NOVEL ALKALINE LIPASE/ESTERASE GENE LIP-1447 DERIVED FROM SOIL METAGENOME AND USE OF SAME

NOVEL ALKALINE LIPASE/ESTERASE GENE LIP-1447 DERIVED FROM SOIL METAGENOME AND USE OF SAME

机译:土壤突变基因组衍生的新型碱性脂酶/酯酶基因LIP-1447及其用途

摘要

The present invention relates to a novel alkaline lipase/esterase gene Lip-1447 derived from soil metagenome, and a use of the same. According to the present invention, a transformant having introduced therein the gene Lip-1447-sub-ORF2 recombined from novel lipase/esterase clone Lip-1447 derived from soil metagenome exhibits potent lipase and esterase activity with respect to tributyrin, and a novel lipase/esterase protein isolated and purified from the transformant also exhibits potent lipase and esterase activities, wherein the novel lipase/esterase protein can be mass-produced from the transformant. Accordingly, the novel lipase/esterase gene, a vector comprising the same, and a transformant having the same introduced therein may be usefully used in the industrial mass-production of a novel lipase/esterase protein with potent activities.;COPYRIGHT KIPO 2020
机译:本发明涉及源自土壤基因组的新型碱性脂肪酶/酯酶基因Lip-1447及其用途。根据本发明,其中导入了由土壤元基因组衍生的新型脂酶/酯酶克隆Lip-1447重组的Lip-1447-sub-ORF2基因重组体,对三丁酸甘油酯具有强力的脂酶和酯酶活性。从转化体分离和纯化的酯酶蛋白还具有有效的脂肪酶和酯酶活性,其中新型的脂肪酶/酯酶蛋白可以从转化体大量生产。因此,新的脂肪酶/酯酶基因,包含该酶的载体以及导入了该酶的转化体可有效地用于具有有效活性的新的脂肪酶/酯酶蛋白的工业批量生产。COPYRIGHT KIPO 2020

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