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Use of thermally annealed multilayer gold nanoparticle films in combination analysis of localized surface plasmon resonance sensing and MALDI mass spectrometry

机译:热退火多层金纳米粒子膜在局部表面等离振子共振传感和MALDI质谱联用分析中的应用

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摘要

A self-assembled film of gold nanoparticles (AuNPs) with a raspberry-like morphology was prepared on a glass plate by the layer-by-layer thermal annealing of multilayer films of AuNPs. It was possible to control the morphology of the obtained films of AuNPs by changing the annealing temperature,duration of annealing, and number of layers. On investigating the plasmonic properties of these films,we found that AuNP films with a raspberry-like morphology yielded the highest refractive index unit,which is a critical parameter in localized surface plasmon resonance (LSPR) sensing, as compared to other types of AuNP films. Self-assembled AuNP films with a raspberry-like morphology were subsequently functionalized with 11-mercaptoundecanoic acid (MUA) to enable the binding of lysozyme to the MUA-modified Au surface. The superior limit of detection for the LSPR sensing of lysozyme in a buffer solution was found to be in the picomolar range (~10(12) M). The high sensitivity observed in the region was attributed to the raspberry-like morphology, where the AuNPs were packed closely together, and the electromagnetic field confinement was most intense (i.e., at hot spots). The MUA-modified, self-assembled AuNP films with a raspberry-like morphology were finally used in the combination analysis of LSPR sensing and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the selective detection and identification of lysozyme in human serum.
机译:通过对AuNPs多层膜进行逐层热退火,在玻璃板上制备了具有树莓样形态的金纳米颗粒(AuNPs)的自组装膜。通过改变退火温度,退火时间和层数,可以控制所获得的AuNPs薄膜的形貌。在研究这些薄膜的等离子体特性时,我们发现与其他类型的AuNP薄膜相比,具有树莓状形态的AuNP薄膜具有最高的折射率单位,这是局部表面等离子体共振(LSPR)传感的关键参数。 。随后将具有树莓状形态的自组装AuNP膜用11-巯基癸酸(MUA)功能化,以使溶菌酶与MUA修饰的Au表面结合。发现缓冲溶液中溶菌酶的LSPR感测的最高检测限为皮摩尔范围(〜10(12)M)。在该区域观察到的高灵敏度归因于树莓状形态,其中AuNP紧密堆积在一起,并且电磁场限制最强烈(即在热点处)。最终,将具有树莓样形态的MUA修饰的自组装AuNP膜用于LSPR传感和基质辅助激光解吸/电离质谱(MALDI-MS)的组合分析,以选择性地检测和鉴定溶菌酶。人血清。

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