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PICH: a DNA translocase specially adapted for processing anaphase bridge DNA

机译:PICH:专门用于处理后期桥DNA的DNA转移酶

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摘要

The Plk1-interacting checkpoint helicase (PICH) protein localizes to ultrafine anaphase bridges (UFBs) in mitosis alongside a complex of DNA repair proteins, including the Bloom's syndrome protein (BLM). However, very little is known about the function of PICH orhow it is recruited to UFBs. Using a combination of microfluidics, fluorescence microscopy, and optical tweezers, we have defined the properties of PICH in an invitro model of an anaphase bridge. We show that PICH binds with a remarkably high affinity to duplex DNA, resulting in ATP-dependent protein translocation and extension of the DNA. Most strikingly, the affinity of PICH for binding DNA increases with tension-induced DNA stretching, which mimics the effect of the mitotic spindle on a UFB. PICH binding also appears to diminish force-induced DNA melting. We propose a model in which PICH recognizes and stabilizes DNA under tension during anaphase, thereby facilitating the resolution of entangled sister chromatids. © 2013 Elsevier Inc.
机译:与Plk1相互作用的检查点解旋酶(PICH)蛋白与包括Bloom's syndrome蛋白(BLM)在内的DNA修复蛋白复合物一起定位于有丝分裂中的超细后期桥(UFB)。但是,对PICH的功能或如何将其招募到UFB知之甚少。通过结合使用微流控技术,荧光显微镜和光学镊子,我们在后期桥的体外模型中定义了PICH的特性。我们显示,PICH以极高的亲和力与双链DNA结合,从而导致ATP依赖性蛋白易位和DNA延伸。最引人注目的是,PICH对结合DNA的亲和力随着张力诱导的DNA拉伸而增加,这模仿了有丝分裂纺锤体对UFB的作用。 PICH结合也似乎减少了力诱导的DNA融合。我们提出了一个模型,在该模型中,PICH可以识别并稳定后期处于张力下的DNA,从而有助于纠缠纠缠的姐妹染色单体。 ©2013爱思唯尔公司。

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