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Gene expression of desaturase (FADS1 and FADS2) and elongase (ELOVL5) enzymes in peripheral blood: association with polyunsaturated fatty acid levels and atopic eczema in 4-year-old children

机译:外周血中去饱和酶(FaDs1和FaDs2)和延伸酶(ELOVL5)酶的基因表达:与4岁儿童的多不饱和脂肪酸水平和特应性湿疹有关

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摘要

Background: It is unknown if changes in the gene expression of the desaturase and elongase enzymes are associated with abnormal n-6 long chain polyunsaturated fatty acid (LC-PUFA) levels in children with atopic eczema (AE). We analyzed whether mRNA-expression of genes encoding key enzymes of LC-PUFA synthesis (FADS1, FADS2 and ELOVL5) is associated with circulating LC-PUFA levels and risk of AE in 4-year-old children. Methods: AE (n=20) and non-AE (n=104) children participating in the Sabadell cohort within the INfancia y Medio Ambiente (INMA) Project were included in the present study. RT-PCR with TaqMan Low-Density Array cards was used to measure the mRNA-expression of FADS1, FADS2 and ELOVL5. LC-PUFA levels were measured by fast gas chromatography in plasma phospholipids. The relationship of gene expression with LC-PUFA levels and enzyme activities was evaluated by Pearson’s rank correlation coefficient, and logistic regression models were used to study its association with risk of developing AE. Results: Children with AE had lower levels of several n-6 PUFA members, dihomo-γ-linolenic (DGLA) and arachidonic (AA) acids. mRNA-expression levels of FADS1 and 2 strongly correlated with DGLA levels and with D6D activity. FADS2 and ELOVL5 mRNA-expression levels were significantly lower in AE than in non-AE children (-40.30% and -20.36%; respectively), but no differences were found for FADS1. Conclusions and Significance: Changes in the mRNA-expression levels of FADS1 and 2 directly affect blood DGLA levels and D6D activity. This study suggests that lower mRNA-expressions of FADS2 and ELOVL5 are associated with higher risk of atopic eczema in young children.
机译:背景:特应性湿疹(AE)儿童的去饱和酶和延长酶的基因表达变化是否与异常的n-6长链多不饱和脂肪酸(LC-PUFA)水平相关是否尚不清楚。我们分析了编码LC-PUFA合成关键酶(FADS1,FADS2和ELOVL5)的基因的mRNA表达是否与循环LC-PUFA水平和4岁儿童发生AE的风险相关。方法:本研究包括参与INfancia y Medio Ambiente(INMA)项目中Sabadell队列的AE(n = 20)和非AE(n = 104)儿童。使用TaqMan低密度阵列卡的RT-PCR测量FADS1,FADS2和ELOVL5的mRNA表达。 LC-PUFA水平通过快速气相色谱法测定血浆磷脂。用皮尔森秩相关系数评估基因表达与LC-PUFA水平和酶活性的关系,并使用逻辑回归模型研究其与发生AE风险的关系。结果:AE儿童的n-6 PUFA成员,二高γ-亚麻酸(DGLA)和花生四烯酸(AA)酸含量较低。 FADS1和2的mRNA表达水平与DGLA水平和D6D活性密切相关。 AE的FADS2和ELOVL5 mRNA表达水平显着低于非AE儿童(分别为-40.30%和-20.36%),但FADS1没有发现差异。结论和意义:FADS1和2的mRNA表达水平的变化直接影响血液DGLA水平和D6D活性。这项研究表明,FADS2和ELOVL5的较低的mRNA表达与幼儿特应性湿疹的较高风险有关。

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