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Evaluation of excretory/secretory products of larval Taenia solium as diagnostic antigens for porcine and human cysticercosis

机译:评价幼虫猪带绦虫的排泄/分泌产物作为猪和人囊尾蚴病的诊断抗原

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摘要

Excretory/secretory antigens (ES) of larval Taenia solium were obtained by maintaining the bladder worms in Medium 199 for 3 days. Analysis by SDS-PAGE showed that ES antigens consisted of at least 19 polypeptides, with M(r) ranging from 14-116 kDa. Analytical isoelectric focusing revealed eight bands with acidic pI. An immunocytolocalization study using the peroxidase method demonstrated the presence of ES epitopes on the tegument of the wall of the spiral canals of bladder worms. The specificity of ES antigens was evaluated by EITB, ELISA and FAST-ELISA using antisera against the common parasites of Chinese pigs and man. ES antigens cross-reacted with the antiserum against larval T. hydatigena of pigs. However, these antigens were generally more specific in diagnosing human cysticercosis. Three host-like molecules with molecular masses 43, 58 and 66 kDa were present in the ES products.
机译:幼虫虫的排泄/分泌抗原(ES)是通过将膀胱蠕虫在199号培养基中放置3天而获得的。通过SDS-PAGE分析显示,ES抗原由至少19个多肽组成,M(r)为14-116kDa。等电分析表明,酸性pI出现了八个谱带。使用过氧化物酶方法进行的免疫细胞定位研究表明,在膀胱蠕虫的螺旋管壁壁上存在ES表位。通过针对中国猪和人常见寄生虫的抗血清,通过EITB,ELISA和FAST-ELISA评估了ES抗原的特异性。 ES抗原与抗猪幼虫T. hydatigena的抗血清交叉反应。但是,这些抗原通常在诊断人囊尾rc病中更具特异性。 ES产物中存在三种分子量为43、58和66kDa的主体样分子。

著录项

  • 作者

    Ko RCC; Ng TF;

  • 作者单位
  • 年度 1998
  • 总页数
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类

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