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Antifungal Activity of Herbal Extracts against Neem Die-Back Pathogen Phomopsis Azadirachtae

机译:中草药提取物对印楝背叶病原菌phodopsis azadirachtae的抗真菌活性

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摘要

Herbal extracts of 26 plants belonging to 20 different families of the plant kingdom were evaluated for antifungal activity against Phomopsis azadirachtae, a fungus causing destructive die-back disease in neem. Test fungus was isolated from infected neem seeds. The screening was performed by poisoned food technique. Extracts of Syzygium aromaticum, Lawsonia inermis, Cinnamomum verum and Allium sativum exhibited significant antifungal activity in a dose-dependent manner. Relatively potent herbal extracts were further evaluated to determine the obligate lowest concentration required to inhibit visible mycelial growth of the pathogen. Syzygium extract exhibited 100% inhibition of the pathogen at minimum concentration of 20% then followed by Lawsonia and Ocimum extracts thus being strongest antifungal agents. The present study revealed that, these plants could be exploited for the possible control of deadly pathogen P. azadirachtae. Accordingly, this is an important step towards preventing the spread of the die-back disease through a more ecofriendly approach.
机译:评估了属于植物界20个不同家族的26种植物的草药提取物对拟南芥(Phomopsis azadirachtae)的抗真菌活性,该菌是引起印em毁灭性死亡的真菌。从受感染的印度em种子中分离出测试真菌。筛选是通过中毒食品技术进行的。香蒲,无核劳氏菌,肉桂和大蒜的提取物均表现出显着的抗真菌活性,且呈剂量依赖性。进一步评估了相对有效的草药提取物,以确定抑制病原体可见菌丝体生长所需的最低最低浓度。蒲桃提取物在最小浓度为20%时对病原体表现出100%的抑制作用,然后是Lawsonia和Ocimum提取物,因此是最强的抗真菌剂。本研究表明,可以利用这些植物来控制致命的病原体印藻。因此,这是通过更生态友好的方法来防止消亡疾病传播的重要步骤。

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