首页> 外文OA文献 >A simplified protocol for detecting two systemic bait markers (Rhodamine B and iophenoxic acid) in small mammals
【2h】

A simplified protocol for detecting two systemic bait markers (Rhodamine B and iophenoxic acid) in small mammals

机译:在小型哺乳动物中检测两种系统性诱饵标志物(若丹明B和碘苯氧酸)的简化方案

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

We developed a method of quantifying levels of fluorescence in the whiskers of wild stoats (Mustela erminea) using fluorescence microscopy and Axiovision 3.0.6.1 software. The method allows for discrimination between natural fluorescence present in or on a whisker, and the fluorescence resulting from the ingestion of the systemic marker Rhodamine B (RB), although some visual judgement is still required. We also developed a new high performance liquid chromatography (HPLC) protocol for detecting the systemic marker iophenoxic acid (IPA) in the blood of laboratory rats (Rattus norvegicus) and wild stoats. With this method, the blood of an animal that has consumed IPA can be tested for the presence of the foreign IPA compound itself. This is a more reliable test than the previous method, which measured the raised level of natural blood protein-bound iodine correlated with IPA absorption. The quantity of blood required from animal subjects is very small (10 μl), so the testing is less intrusive and the method can be extended to smaller species. The extraction technique uses methanol, rather than acids and heavy metal salts, thereby simplifying the procedure. Recovery of IPA is quantitative, giving a highly reliable reading. In experiments on captive rats the IPA method proved successful. Of 12 positively marked carcasses, two that had not been frozen for the 24 h before blood samples were taken showed relatively lower IPA levels. The same IPA detection method, as well as the whisker analysis for RB, was applied successfully to a population of wild stoats to which both Rhodamine B and IPA were made available at bait stations. The presence of both bait markers was detectable in rats for at least 21 days and in stoats for at least 27 days.
机译:我们开发了一种使用荧光显微镜和Axiovision 3.0.6.1软件量化野生白须(Mustela erminea)晶须中荧光水平的方法。该方法可以区分晶须中或晶须上存在的自然荧光,以及由于摄入全身性标记若丹明B(RB)而产生的荧光,尽管仍然需要一些视觉判断。我们还开发了一种新的高效液相色谱(HPLC)方案,用于检测实验大鼠(Rattus norvegicus)和野生动物血液中的系统性标记物碘苯氧酸(IPA)。使用这种方法,可以检测食用IPA的动物血液中是否存在外来IPA化合物本身。这是比以前的方法更可靠的测试,以前的方法测量了与IPA吸收相关的天然血液结合的碘水平的升高。动物受试者所需的血液量很小(10μl),因此测试的侵入性较小,该方法可以扩展到较小的物种。萃取技术使用甲醇,而不是酸和重金属盐,从而简化了过程。 IPA的回收是定量的,可提供高度可靠的读数。在圈养大鼠的实验中,IPA方法证明是成功的。在12个阳性标记的屠体中,有两个在采血前24小时未冷冻的屠体显示出较低的IPA水平。相同的IPA检测方法以及RB的晶须分析已成功应用于诱饵站提供罗丹明B和IPA的野生种群。两种诱饵标记物的存在在大鼠中至少检测了21天,在肥大中检测了至少27天。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号