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PSN-PC: A Novel Antimicrobial and Anti-Biofilm Peptide from the Skin Secretion of Phyllomedusa-camba with Cytotoxicity on Human Lung Cancer Cell

机译:PSN-PC:Phyllomedusa-camba皮肤分泌物对人肺癌细胞具有细胞毒性的新型抗菌和抗生物膜肽

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摘要

Peptides derived from amphibian skin secretion are promising drug prototypes for combating widespread infection. In this study, a novel peptide belonging to the phylloseptin family of antimicrobial peptides was isolated from the skin secretion of the Phyllomedusa camba, namely phylloseptin-PC (PSN-PC). The biosynthetic precursor was obtained by molecular cloning and the mature peptide sequence was confirmed through tandem mass spectrometry (MS/MS) fragmentation sequencing in the skin secretion. The synthetic replicate exhibited a broad spectrum antimicrobial activity against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans at concentrations of 2, 2, 8, 32 and 2 µM, respectively. It also showed the capability of eliminating S. aureus biofilm with a minimal biofilm eradication concentration of 8 µM. The haemolysis of this peptide was not significant at low concentrations but had a considerable increase at high concentrations. Additionally, this peptide showed an anti-proliferation effect on the non-small cell lung cancer cell line (NCI-H157), with low cytotoxicity on the human microvascular endothelial cell line (HMEC-1). The discovery of the novel peptide may provide useful clues for new drug discoveries.
机译:来自两栖动物皮肤分泌的肽是用于对抗广泛感染的有希望的药物原型。在这项研究中,从毛毛楠的皮肤分泌物中分离出一种新的属于抗菌肽phylloseptin家族的肽,即phylloseptin-PC(PSN-PC)。通过分子克隆获得生物合成的前体,并通过皮肤分泌物中的串联质谱(MS / MS)片段测序确定成熟的肽序列。合成的复制品分别对金黄色葡萄球菌,耐甲氧西林的金黄色葡萄球菌,大肠杆菌,铜绿假单胞菌,白色念珠菌具有广谱抗菌活性。浓度分别为2、2、8、32和2 µM。它还显示了消除金黄色葡萄球菌生物膜的能力,最小的生物膜根除浓度为8 µM。该肽在低浓度时的溶血作用不明显,但在高浓度时的溶血作用明显增加。另外,该肽对非小细胞肺癌细胞系(NCI-H157)显示出抗增殖作用,对人微血管内皮细胞系(HMEC-1)具有低细胞毒性。新肽的发现可能为新药发现提供有用的线索。

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