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Obese gene expression: reduction by fasting and stimulation by insulin and glucose in lean mice, and persistent elevation in acquired (diet-induced) and genetic (yellow agouti) obesity.

机译:肥胖的基因表达:瘦小鼠的禁食和胰岛素和葡萄糖刺激使其减少,以及获得性(饮食诱发)和遗传性(黄色刺鼠)肥胖持续升高。

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摘要

Mutations in the obese (ob) gene lead to obesity. This gene has been recently cloned, but the factors regulating its expression have not been elucidated. To address the regulation of the ob gene with regard to body weight and nutritional factors, Northern blot analysis was used to assess ob mRNA in adipose tissue from mice [lean, obese due to diet, or genetically (yellow agouti) obese] under different nutritional conditions. ob mRNA was elevated in both forms of obesity, compared to lean controls, correlated with elevations in plasma insulin and body weight, but not plasma glucose. In lean C57BL/6J mice, but not in mice with diet-induced obesity, ob mRNA decreased after a 48-hr fast. Similarly, in lean C57BL/6J controls, but not in obese yellow mice, i.p. glucose injection significantly increased ob mRNA. For up to 30 min after glucose injection, ob mRNA in lean mice significantly correlated with plasma glucose, but not with plasma insulin. In a separate study with only lean mice, ob mRNA was inhibited >90% by fasting, and elevated approximately 2-fold 30 min after i.p. injection of either glucose or insulin. These results suggest that in lean animals glucose and insulin enhance ob gene expression. In contrast to our results in lean mice, in obese animals ob mRNA is elevated and relatively insensitive to nutritional state, possibly due to chronic exposure to elevated plasma insulin and/or glucose.
机译:肥胖(ob)基因突变会导致肥胖。最近已经克隆了该基因,但尚未阐明调节其表达的因素。为了解决ob基因在体重和营养因素方面的调节问题,使用Northern印迹分析来评估不同营养条​​件下小鼠[瘦,因饮食而肥胖或遗传性(黄色刺豚鼠)肥胖]小鼠脂肪组织中的ob mRNA条件。与瘦对照相比,两种肥胖形式的ob mRNA均升高,与血浆胰岛素和体重的升高相关,但与血浆葡萄糖的升高无关。在瘦的C57BL / 6J小鼠中,但在饮食引起的肥胖的小鼠中却没有,ob基因在禁食48小时后下降。类似地,在瘦的C57BL / 6J对照中,但在肥胖的黄色小鼠中则没有。葡萄糖注射明显增加了ob mRNA。葡萄糖注射后长达30分钟,瘦小鼠中的ob mRNA与血浆葡萄糖显着相关,但与血浆胰岛素无关。在另一只只用瘦小鼠的研究中,禁食可将ob mRNA抑制> 90%,并在腹膜后30分钟升高2倍。注射葡萄糖或胰岛素。这些结果表明,在瘦肉动物中,葡萄糖和胰岛素增强了ob基因的表达。与我们在瘦小鼠中的结果相反,在肥胖动物中,ob mRNA升高且对营养状态相对不敏感,这可能是由于长期暴露于升高的血浆胰岛素和/或葡萄糖引起的。

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