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A major pathogenic antigen of Heymann nephritis is present exclusively in the renal proximal tubule brush border--studies with a monoclonal antibody against pronase-digested tubular antigen.

机译:Heymann肾炎的主要病原体抗原仅存在于肾小管刷缘附近-研究是针对链霉菌消化的肾小管抗原的单克隆抗体。

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摘要

We have isolated a nephritogenic 120-kD antigen from rat renal tubule brush border that induces rat Heymann nephritis. A MoAb that recognized this antigen reacted exclusively with the brush border on indirect immunofluorescence and immunoelectron microscopy. Rabbit antiserum against this antigen also reacted exclusively with the brush border. With the injection of this antiserum, rabbit IgG became detectable along the glomerular basement membrane (GBM) after 3 days. Our 120-kD antigen was shown to have a close relationship with gp330 based on the following: (i) this antigen can induce active Heymann nephritis as gp330; (ii) our MoAb reacted with the immune deposits of nephritic kidneys induced not only by the 120-kD antigen but also by gp330, and conversely, rabbit antiserum against gp330 reacted with those induced by the 120-kD antigen as well as gp330; and (iii) by immunoblotting, polyclonal antibodies against the 120-kD antigen reacted with gp330 and polyclonal antibodies against gp330 reacted with the 120-kD antigen. These observations indicate that antigen present exclusively in the brush border can induce active Heymann nephritis, and the common antigenic determinants shared by brush border and the coated pits of glomerular epithelium may not be a prerequisite to induce nephritis. A more precise relationship between the 120-kD antigen and reported C14 fusion protein or 40-kD alpha 2MRAP remains to be established.
机译:我们从大鼠肾小管刷缘中分离出了一种致肾炎的120 kD抗原,可诱导大鼠Heymann肾炎。在间接免疫荧光和免疫电子显微镜下,识别该抗原的MoAb仅与刷状边缘反应。兔针对这种抗原的抗血清也仅与刷状边缘反应。通过注射这种抗血清,三天后沿肾小球基底膜(GBM)即可检测到兔IgG。我们的120 kD抗原与gp330具有密切关系,其基于以下方面:(i)这种抗原可以像gp330一样诱发活动性Heymann肾炎; (ii)我们的单抗(MoAb)与不仅由120 kD抗原而且由gp330诱导的肾肾脏的免疫沉积物发生反应,反之,针对gp330的兔抗血清与由120 kD抗原和gp330诱导的兔抗血清反应; (iii)通过免疫印迹,针对与gp330反应的120kD抗原的多克隆抗体和针对与120kD抗原反应的gp330的多克隆抗体。这些观察结果表明,仅存在于刷状缘中的抗原可诱发活动性海曼肾炎,并且刷状缘和肾小球上皮的包膜凹坑共有的常见抗原决定簇可能不是诱发肾炎的先决条件。 120 kD抗原与已报道的C14融合蛋白或40 kD alpha 2MRAP之间的更精确关系仍有待建立。

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