Isolation of a Novel Thermophilic Anoxybacillus flavithermus SO-13, Production, Characterization and Industrial Applications of its Thermostable alpha-Amylase

摘要

In this research paper, a novel thermophilic bacteria named strain SO-13 was isolated from hot spring mud sample udin Afyonkarahisar (Omer). Based on morphological and biochemical tests, 16S rRNA gene sequence analysis, udG-C content and DNA-DNA hybridization analysis, the thermophilic isolate belonged to Anoxybacillus genus and it udwas closely related to A. flavithermus. The effects of different solid state fermentation conditions such as different udsubstrate, incubation time, temperature, pH and surfactants on α-amylase production were experimented. In addition udto these, different parameters such as temperature and temperature stability, pH and pH stability, detergents and udsurfactants, different starches and metal ions on effect of partially purified enzyme characterization were examined. udThe optimum temperature and pH of thermostable α-amylase was 80°C and 7.0, respectively. Thermostable udα-amylase hydrolyzed the 76% and 87% of soluble starch content in red and green apple juice at 80°C in 30 min