BACKGROUNDududCentrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.ududAIMududDetermine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses.ududMETHODSududNuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.ududRESULTSududBesides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.ududCONCLUSIONududAlready minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
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机译：背景 ud ud离心是血浆样品制备过程中必不可少的步骤，但是实验室之间的应用条件可能会有所不同。 ud udAIM ud ud确定常规使用的血浆离心方案（1500×g 10分钟; 3000×g 5分钟）是否会产生影响非目标代谢组学分析。 ud ud除了血小板计数方面的显着差异外，我们还发现了与不同离心方案相关的NMR和HRMS数据的实质性变化。 ud ud结论 ud ud血浆离心中的微小差异已经可以显着影响代谢组学模式，并可能影响代谢组学研究。
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