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Extended-spectrum ß-lactamase, AmpC-producing, and fluoroquinolone-resistant Escherichia coli in retail broiler chicken meat, Italy

机译:意大利零售肉鸡鸡肉中的广谱β-内酰胺酶,产生AmpC的和耐氟喹诺酮的大肠杆菌

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摘要

Background: Globally, antimicrobial drug-resistant Escherichia coli is among the most common etiological agents of invasive disease in humans. In Europe, increasing proportions of infections due to third-generation cephalosporins and/or fluoroquinolone-resistant extraintestinal pathogenic E. coli (ExPEC) strains are reported. E. coli from poultry are those more closely linked to human E. coli, but lack of reliable data makes it difficult to assess the attributable risk of different food sources. In the present study, our objective was to investigate the antimicrobial resistance profile, phylogenetic background, and virulence factors of E. coli isolates from broiler chicken meat sold at retail in Palermo, Italy. Materials and Methods: Isolation of multidrug resistant (MDR) E. coli was performed during April-December 2013 on a total of 163 chicken meat samples. Susceptibility to a panel of nine antimicrobial agents was determined. PCR assays were carried out to detect extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase, and plasmid-mediated quinolone resistance (PMQR) genes, phylogenetic group, and ExPEC-associated traits. A single nucleotide polymorphism (SNP) PCR was done to detect E. coli sequence type (ST)131. Results: One hundred thirty-four isolates from 109 meat samples were MDR. B1 was the most prevalent phylogenetic group (47.8%), followed by groups D (25.4%), A (22.3%), and B2 (4.5%). ESBLs and AmpC β-lactamases were detected by PCR in 132 (98.5%) and 15 (11.2%) isolates. PMQR determinants were detected in 122 (91%) isolates. Twenty-two MDR isolates met the molecular definition of ExPEC. SNP-PCR results confirmed that four B2 isolates were ST131. Enterobacterial Repetitive Intergenic Consensus sequence-PCR analysis showed a large heterogeneity with 55 unique profiles and 31 clusters including 2-4 isolates. Conclusions: An alarmingly high prevalence of MDR E. coli from broiler chicken meat is evident in our geographic area. The ongoing use of antimicrobial drugs in livestock should be urgently restricted, particularly in the poultry sector.
机译:背景:全球范围内,抗菌药物耐药的大肠杆菌是人类最常见的侵袭性疾病病原体之一。在欧洲,据报道,由于第三代头孢菌素和/或对氟喹诺酮类耐药的肠外致病性大肠杆菌(ExPEC)菌株引起的感染比例增加。家禽中的大肠杆菌与人大肠杆菌之间的联系更紧密,但是缺乏可靠的数据使得难以评估不同食物来源的可归因风险。在本研究中,我们的目的是调查在意大利巴勒莫零售的肉鸡大肠杆菌分离株的抗菌素耐药性,系统发育背景和致病因子。材料和方法:2013年4月至12月,对多药耐药(MDR)大肠杆菌进行了分离,共采集了163个鸡肉样品。确定了对一组九种抗菌剂的敏感性。进行了PCR检测,以检测广谱β-内酰胺酶(ESBL),质粒介导的AmpCβ-内酰胺酶和质粒介导的喹诺酮抗性(PMQR)基因,系统发生群和与ExPEC相关的性状。进行了单核苷酸多态性(SNP)PCR以检测大肠杆菌序列类型(ST)131。结果:从109份肉样品中分离出了一百三十四份MDR。 B1是最普遍的系统发育组(47.8%),其次是D组(25.4%),A组(22.3%)和B2(4.5%)。通过PCR在132(98.5%)和15(11.2%)分离株中检测到ESBLs和AmpCβ-内酰胺酶。在122个(91%)分离物中检测到PMQR决定因素。 22个MDR分离物符合ExPEC的分子定义。 SNP-PCR结果证实了四个B2分离株是ST131。肠细菌重复基因间共识序列-PCR分析显示具有55个独特特征和31个簇(包括2-4个分离株)的较大异质性。结论:在我们的地理区域内,肉鸡MDR大肠杆菌的流行率惊人地高。应紧急限制在牲畜中持续使用抗菌药物,特别是在禽类部门。

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