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Human telomerase reverse transcriptase expression in oral carcinogenesis - a preliminary report

机译:人端粒酶逆转录酶表达在口腔癌变中的作用-初步报告

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摘要

Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, is strongly associated with telomerase activity implicated in cellular immortalization and tumorigenesis. In situ detection of hTERT will aid in determining the localization of telomerase positive cells. The aim of this study was to detect hTERT protein expression in multistep oral carcinogenesis using paraffin embedded tissue samples, and to study the relationship of hTERT expression with different histological stages in oral carcinogenesis. Normal (n=4), hyperplastic (n=4), dysplastic (n=4) and neoplastic (n=10) oral epithelia representing different histological stages in oral carcinogenesis were included in the study. hTERT protein detection was done by immunohistochemistry (IHC) technique. Nuclear staining intensities were noted and the hTERT-labelling index was determined. Dysplastic and neoplastic oral epithelia showed an increased percentage of hTERT positive cells (Grade 4: >50 positive staining nuclei) with intense staining in the basal, parabasal and superficial layers of the epithelia, unlike normal oral mucosa which showed intense staining only in the basal and parabasal cell layers, which are the normal proliferative progenitor compartments. hTERT protein expression was elevated with the corresponding advancement of the histological stages of oral carcinogenesis, from normal to hyperplasia to dysplasia to carcinoma. There seems to be an upregulation of hTERT protein expression during the progression of oral cancer, therefore, this may indicate the feasibility of IHC detection of hTERT protein in oral carcinogenesis as a potential diagnostic or prognostic marker.
机译:人端粒酶逆转录酶(hTERT)是端粒酶的催化亚基,与端粒酶活性密切相关,端粒酶活性与细胞永生化和肿瘤发生有关。 hTERT的原位检测将有助于确定端粒酶阳性细胞的定位。这项研究的目的是使用石蜡包埋的组织样本检测hTERT蛋白在多步口腔癌变过程中的表达,并研究hTERT表达与口腔癌变过程中不同组织学阶段之间的关系。正常(n = 4),增生(n = 4),增生(n = 4)和赘生性(n = 10)口腔上皮细胞代表了口腔癌发生过程中不同的组织学阶段。 hTERT蛋白检测是通过免疫组织化学(IHC)技术进行的。记录核染色强度并确定hTERT标记指数。与正常的口腔粘膜仅在基底膜上显示强烈染色不同,口腔发育不良和肿瘤的上皮显示出百分比的hTERT阳性细胞百分比增加(4级:> 50个阳性染色细胞核),在上皮的基底,副基底和浅表层有强烈染色。和副基底层,这是正常的增殖祖细胞室。从正常到增生再到异常增生再到癌,口腔癌发生的组织学阶段相应地发展,hTERT蛋白的表达也随之升高。在口腔癌的进展过程中,hTERT蛋白的表达似乎上调,因此,这可能表明在口腔癌发生过程中用IHC检测hTERT蛋白作为潜在的诊断或预后指标的可行性。

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