Biolistic and agrobacterium-mediated genetic transformation of immature and mature embryos of spring wheat cultivar Saratovskaya-29

摘要

Plant transformation provides a promising methodology of introducing new genes thatencode desirable traits to a wide range of crop plants. Success in genetic transformationhas been achieved in many of the important crop species, such as soybean, cotton, rice,corn. However, wheat, one of the major crops of the world, has been considered to bedifficult to transform via either Agrobacterium or biolistic bombardment (Rakszegi etal., 2001). There have been limited studies on A. tumefaciens-mediated transformation ofcereals, including wheat, because of the overall refractory character of host-pathogeninteractions between Agrobacterium and the cereal plants (Gould et al., 1991; Hiei et al.,1994; Cheng et al., 1997). While the genetic transformation of rice usingAgrobacterium has become routine, only a few successful studies of Agrobacterium-mediated transformation of wheat have been reported, and these involved a model springwheat, Triticum aestivum cultivar Bobwhite (Cheng et al., 1997). Model genotypes aredeveloped for ease of plant regeneration in tissue culture and both Agrobacterium andbiolistic mediated transformation methods require regeneration of plants in tissueculture. More success has been achieved in obtaining fertile transgenic wheat plants byparticle bombardment, or biolistics method (Vasil et al., 1992; Weeks et al., 1993;Becker et al., 1994; Zhou et al., 1995; Altpeter et al., 1996). Wheat plants of the modelsystem cultivar Bobwhite were used in most of these studies as well. The primary objective of this study was to use the callus-based transformation procedures mentioned above with a non-model cultivar of hexaploid spring wheat Saratovskaya-29, widely grown in Kazakhstan, to test the genotype dependence of the previously developed transformation protocols with respect to stable transfer of DNA and regeneration of transgenic plants. The spring wheat cultivar Saratovskaya-29 (Albidum-24/ Lutescens-55-11) was chosen for the study as being one of the most widely grown wheat cultivars both in Russia and Kazakhstan. It was bred in early 50??s in the Research Institute of the South-East, Saratov. Because of its drought resistance and good baking quality traits, Saratovskaya-29 reached a peak of nearly 21.2 mln ha in the former USSR in 1996 (Martynov and Dobrotvorskaya, 1996). Economical importance of this cultivar makes it an appropriate candidate for further improvement of economically significant traits. Another objective of the study described was to compare the transformation efficiencies and inheritance in the transgenic plants produced.