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Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay

机译:使用荧光微球免疫色谱试验条测定的米粉中米粉中Ochratoxina的快速和敏感性检测

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摘要

In this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant parameters were optimized including the best concentration of coating antigen and optimal diluent buffer composition. Under optimal conditions, the strip assay had a visual limit of detection (LOD) of 2 ng/mL and a cut-off value of 16 ng/mL for OTA in rice flour samples. By means of a strip scan reader, we obtained a quantitative measurement for OTA with an LOD of 1.27 ng/mL. No significant difference in the strip test results was seen when compared to an ic-ELISA assay or LC–MS/MS. This also shows, therefore, that the FM-ICTS has potential application for rapid and accurate quantitative detection in the market place.
机译:在该研究中,基于Ochratoxina(OTA)的羟基和羧基末端,开发了高灵敏度和特异性单克隆抗体(MAB)。组装荧光微球免疫色谱试验条(FM-ICT)以快速检测米粉中的OTA。优化了一些相关参数,包括最佳浓度的涂层抗原和最佳稀释剂缓冲组合物。在最佳条件下,条带测定具有2ng / ml的检测(LOD)的视觉极限,并且在米粉样品中对于OTA的截止值为16ng / ml。借助于条带扫描读取器,我们获得了OTA的定量测量,LOD为1.27ng / ml。与IC-ELISA测定或LC-MS / MS相比,可以看到条带测试结果的显着差异。因此,这也表明,FM-ICT在市场上具有潜在的应用,可在市场上进行快速准确的定量检测。

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