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Functional Validation of a New Alginate-based Hydrogel Scaffold Combined with Mesenchymal Stem Cells in a Rat Hard Palate Cleft Model

机译:新型藻酸盐基水凝胶支架的功能验证与大鼠硬腭裂解模型中的间充质干细胞联合

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摘要

Background:. One of the major difficulties in cleft palate repair is the requirement for several surgical procedures and autologous bone grafting to form a bony bridge across the cleft defect. Engineered tissue, composed of a biomaterial scaffold and multipotent stem cells, may be a useful alternative for minimizing the non-negligible risk of donor site morbidity. The present study was designed to confirm the healing and osteogenic properties of a novel alginate-based hydrogel in palate repair.Methods:. Matrix constructs, seeded with allogeneic bone marrow–derived mesenchymal stem cells (BM-MSCs) or not, were incorporated into a surgically created, critical-sized cleft palate defect in the rat. Control with no scaffold was also tested. Bone formation was assessed using microcomputed tomography at weeks 2, 4, 8, and 12 and a histologic analysis at week 12.Results:. At 12 weeks, the proportion of bone filling associated with the use of hydrogel scaffold alone did not differ significantly from the values observed in the scaffold-free experiment (61.01% ± 5.288% versus 36.91% ± 5.132%; p = 0.1620). The addition of BM-MSCs stimulated bone formation not only at the margin of the defect but also in the center of the implant.Conclusions:. In a relevant in vivo model of cleft palate in the rat, we confirmed the alginate-based hydrogel’s biocompatibility and real advantages for tissue healing. Addition of BM-MSCs stimulated bone formation in the center of the implant, demonstrating the new biomaterial’s potential for use as a bone substitute grafting material for cleft palate repair.
机译:背景:。腭裂修复的主要困难之一是需要几种外科手术和自体骨移植,以形成横跨裂缝缺陷的骨桥。由生物材料支架和多能干细胞组成的工程化组织可以是最小化供体现场发病率的不可忽略的风险的有用替代方案。本研究旨在确认新型藻酸盐的水凝胶在口感修复中的愈合和成骨特性。方法:将与同种异体骨髓衍生的间充质干细胞(BM-MSCs)播种的基质构建体掺入大鼠外科造成的临界大小的腭裂缺陷中。还测试了没有支架的对照。在第2,4,8和12周使用微型仿层析术评估骨形成,并在第12周的组织学分析。结果:在12周时,单独使用水凝胶支架相关的骨填充的比例从无支腿实验中观察到的值没有显着差异(61.01%±5.288%,与36.91%±5.132%; p = 0.1620)。添加BM-MSCs刺激骨骼形成不仅在缺陷的边缘处,而且刺激骨骼形成,而且在植入物的中心。结论:。在大鼠中腭裂的体内模型中,我们证实了藻酸盐的水凝胶的生物相容性和基于组织愈合的实际优势。添加BM-MSCs刺激植入中心的骨形成,展示了新的生物材料用作骨替代植物修复的骨替代植物修复。

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