Introduction and Study Design (Evaluation of Four Plant Bioassays for DetectingGenetic Hazards of Environmental Chemicals)

摘要

Under the sponsorship of the UNEP/ILO/IPCS, 17 laboratories from diverse regionsof the world participated in evaluating the utility of four plant bioassays for detecting genetic hazards of environmental chemicals. The bioassays included in this collaborative study were Arabidopsis embryo and chlorophyll assay and Tradescantia stamen hair assay. Tradescantia micronucleus assay, and Vicia faba root tip assay. Four to six laboratories participated in the conduction of each of the bioassays. All laboratories participating in a particular bioassay were supplied with uniform plant material as well as a standardized protocol. Five direct acting water soluble test chemicals, i.e., maleic hydrazide, methyl nitrosourea, ethyl methanesulfonate, sodium azide, and azidoglycerol were selected for this study. The study was designed to be completed in three phases. Ethyl methanesulfonate was used as a positive control and has already been reported earlier (Sandhu et al., 1991). The data from the remaining four chemicals used for the evaluation of four plant test systems in first phase of the collaborative study are reported in this volume. (Copyright (c) 1994 Elsevier Science B.V.)