Development of High Titer Antigens, Killed Vaccines and Specific Antisero Consonant with the Research and Development of Rhinoviruses

摘要

Four, fifty liter lots of rhinoviruses have been propagated in WI cells. After appropriate concentration and purification, vaccines will be prepared of each lot for use in man. Normal chromosome patterns were found in three of the four lots of WI cells, and the vaccines produced from these normal cells are in various stages of safety testing. Studies designed to evolve an animal potency test indicate that comparable antibody response can be produced in guinea pigs injected with two, five, or ten ml of rhinovirus vaccine. The potency of a rhinovirus vaccine to guinea pigs was not significantly affected by the dosage schedule. The immunizing activity of rhinovirus vaccines could be concentrated by vacuum dialysis or high speed centrifugation. No stimulation of neutralizing antibody was observed in human volunteers given an inactivated and unconcentrated 33342 vaccine. The vaccine had been shown to be potent in guinea pigs. Further work with this vaccine will be carried out after the virus is concentrated.