Preparation of Immune Serum Serum to Enteroviruses

摘要

Eight enterovirus fluids intended for use as reference seed and concentrated antigens were tested for virus infectivity and identity. These were coxsackieviruses A3, A16, and echoviruses 15, 21, 29, 30, and 31. The tests were satisfactory and the concentrated antigens were released for use in the immunization of horses. The release of the echovirus antigens completes the projected program for preparation of echovirus immune serum in horses. Certification tests for infectivity, identity and purity were completed on seven ampuled reference seeds (poliovirus 1 Mg-stabilized; poliovirus 2 original fluid, and Mg-stabilized; coxsackievirus B2 original fluid; coxsackievirus A9 Mg-stabilized; and echoviruses 11, 14 Mg-stabilized). All were satisfactory except coxsackievirus A9, which was found to be contaminated with poliovirus 1. A purified seed stock was prepared and is recommended for use in the preparation of a new lot of reference seed. Eighteen additional ampuled reference seeds for certification were received. These are coxsackieviruses A2, 8, 10, 11, 12, 13, 16, 20a, and echovirus 21 (propagated in human amnion cells) and echovirused 9, 13, 15, 26, and 29 (propagated in monkey kidney). (Author)