PREPARATION OF IMMUNE SERUM TO ENTEROVIRUSES.

摘要

Certification tests for infectivity, identy and purity were completed on 10 ampuled reference seeds. These were the original fluids of coxsackieviruese A3and A20a and Mg-stabilized seeds of coxsackieviruses A2, A8, A10-A12, A20a and echoviruses 21and 29. Two seeds were low in infectivity titer, coxsackievirus A3 (original fluid) and coxsackievirus A2Mg-stabilized. The rest were satisfactory in infective titer and purity. Submission of samples of pooled and dried enterovirus horse sera continued through this period as the materials have become available from Center Laboratories. Tests for homologous antibody were completed on 8sets (coxsackieviruses A1, A5, A6, A12, A22and echoviruses 13, 14and 23). Results showed that the titers were within the range predicted from tests on the bleedings recommended for pooling. Sixteen sets are awaiting tests: coxsackieviruses A2, A8, A10, A11, A13, A16, A20and echoviruses 15, 24-27, 29-32. Tests for homologous antibody were completed on the bleedings of 5series of exsanguinated horses (coxsackieviruses A11, A14, A15, A19and echovirus 33). Horses immunized with coxsackieviruses A3, A17, A18, A21, A24have been exsanguinated and a series of trial mixtures are under test. The two animals inoculated with coxsackievirus A4failed to respond to the repeated immunixing and properly spaced booster doses,and have been discarded. Recommendations have been made to start a new series of animals using preferably mouse brain-torso antigen. (Author)