Transplacental and Translactational Distribution of Radiolabeled N-OH-2-Acetylaminofluorene in the BALB/c Mouse

摘要

Experiments were conducted to study the transplacental and translactational passage and distribution of radiolabeled N-OH-2-AAF in the BALB/c mouse. The radiolabeled N-OH-2-AAF radily crossed the placenta to the fetuses when the compound was administered by oral gavage to pregnant BALB/c mice during late gestation. The radioactivity from the (3H)-N-OH-2-AAF was detected in the fetuses as early as 1 hour after the administration of the compound to the maternal animal. At five hours radioactivity in the fetuses was greater than that observed at 1 hour and at 24 hours the radioactivity was only slightly reduced. Radioactivity was detected in maternal tissues at 1 hour and was greater at 5 hours but the radioactivity at 24 hours was greatly diminished when compared to the activity at 1 hour. The activity was detected in the liver, kidney, and bladder tissues in both the maternal and fetal animals with slightly higher concentrations being found in the bladder than in the other tissues. Lactational transfer of radiolabeled N-OH-2-AAF was minimal if parturition occurred 48 hours after the last administration of the compound.