Lignin Degradation and Lignin Peroxidase Production in Cultures of 'Phanerochaetechrysosporium' Immobilized on Porous Ceramic Supports

摘要

The ligninolytic fungus Phanerochaete chrysosporium has been proposed as abiocatalyst for the degradation of aromatic pollutants in wastewaters. Application of the organism for this purpose will benefit from improved methods for immobilization in culture. In the investigation, P. chrysosporium was grown in rotary-shaken immobilized culture on 1.3-cm diameter porous alumina spheres. These cultures degraded a synthetic (14)C-labeled guaiacyllignin at the same rate that conventional nonimmobilized cultures did, and produced ligninolytic peroxidases at levels comparable to those previously reported for nonimmobilized agitated cultures. Immobilization of the fungus greatly facilitated periodic replacement of the extracellular medium, and the porous supports were reusable after removal of the spent mycelium. (Copyright (c) 1990 Butterworth-Heinemann.)