Interrelations of Transformation, Virus Replication, and Differentiated Functions in Cultured Chick Cells

摘要

Previous studies demonstrated that chick tendon cells can be transformed by Rous sarcoma virus as judged by standard criteria of altered morphology, loss of density-dependent growth control and increase in 2-deoxy-D-glucose uptake and that collagen synthesis by these cells drops drastically upon transformation. To study the time course of this event, the change in collagen synthesis after transformation by a temperature-sensitive mutant of the virus was followed. This change is then compared to changes in other cellular properties. The mechanism by which collagen synthesis is regulated after transformation has also been investigated in this thesis. The effect of 2-deoxy-D-glucose on the cellular metabolism of chick embryo fibroblasts as well as on the replication of Rous sarcoma virus in infected chick cells was studied in this project. A possible relationship between host cellular metabolism and virus replication was also explored. (ERA citation 05:009311)