首页> 美国政府科技报告 >Restore Wild-Type Functions to P53 Mutants Using an RNA- Based Combinatorial Approach
【24h】

Restore Wild-Type Functions to P53 Mutants Using an RNA- Based Combinatorial Approach

机译:使用基于RNa的组合方法将野生型功能恢复为p53突变体

获取原文

摘要

The tumor suppressor p53 plays pivotal roles in maintaining the integrity of the genome and regulates cell cycle arrest and programmed cell death (apoptosis). Tumors without a functioning p53 are more likely to have a very aggressive clinical course. We proposed to use an RNA-based combinatorial approach to select for single-stranded RNA molecules that will bind to a mutant p53 protein, correct its conformation, and restore its sequence-specific DNA- binding activity. We have successfully accomplished the tasks we proposed for the first year of funding, including (1) expression and purification of human wild-type p53 protein in insect cells, (2) in vitro selection of RNA aptamers from a pool of RNA molecules that bind to p53 protein, and (3) sequence-specific DNA binding assays using the EMSA technique. Sequence and structural analysis of the selected RNAs revealed that the ability of RNA molecules to interfere with sequence-specific DNA binding of p53 may depend on their tertiary structures.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号