Effect pf Estrogen on Progression of Human Proliferation Breast Cancer Disease in a Xenograft Model

摘要

Utilizing the T24-Ha-ras transfected MCFlOA xenograft model of early human breast cancer progression we have (1) demonstrated the effects of tamoxifen on growth and sequence of progression of human preneoplastic breast disease, (2) validated morphological indices of progression with expression and localization of bcl-2, bax, c-erbB2 and Ki67, (3) established a three dimensional in vitro assay system that recapitulates several important aspects of estrogen-induced growth and preneoplastic progression of MCF10AT1 cells in vivo, and demonstrates for the first time the integral role endothelial cells play in ductal-alveolar morphogenesis and proliferation of preneoplastic human breast epithelial cells.