Roles of Breast Cancer Genes in DNA Homologues Recombination and Cellular Sensitivity to Radiation and Anticancer Drugs

摘要

The purpose of this research is to investigate the roles of RAD5 1- interacting breast cancer genes in homologous recombination and cellular resistance to anti-cancer drugs. We outlined 5 specific tasks in the original proposal. The first year of the project mainly involved tasks 1 and 5. Task 1 was designed to establish reliable over-expressing cell systems in mammalian cells. These cells will be used in tasks 2, 3, and 4 starting in the second year of the project. Without changing the objectives of the study, we have made improvement in the technical approaches toward the goal of this task, and significant progress was made in the first year of research. The purpose of task 5 was to identify novel BRCA interacting proteins. In the first year, we planned to complete the yeast two hybrid screen process, and we have accomplished it. Two new genes were identified by a yeast two-hybrid screening.