Genetic Induction of Cultured Human Cells by Femtosecond Pulse Laser- Light Exposure

摘要

The use of laser light for targeting devices and weapons has sharply increased the likelihood that aircrew and support personnel will be exposed during operations. The increased potential for exposure of humans to laser light highlights the need for scientifically-based safety standards for laser exposure at the ultrashort pulse lengths. Current safety standards are largely extrapolations of exposure limits at longer pulse lengths using a minimal visible lesion endpoint in the Rhesus monkey retinal model. A non-animal model for assessing laser-light damage to tissue, particularly human, is quite desirous for obvious scientific, political, and fiduciary reasons. We assessed the sublethal insult to human cells using a tissue culture system for specific genes that have been shown to be important in several biological processes that could lead to cancer or cell death. Using the CAT-Tox (L) assay, it appears that 910 and 455 nm, femtosecond pulses of laser light is sensed and induces several stress response genes. Both wavelengths induced the xenobiotic receptor element in a roughly dose dependent fashion at 48 hours post exposure; while also inducing other genes to yield a genetic expression fingerprint unique to the exposure parameters. This approach provides insight into a more global methodology for characterizing environmental stressors via genetic profiling.